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murJ [2018-04-03 18:50:14]
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murJ [2018-04-03 18:50:14]

lipid II flippase
Locus
BSU30050
Isoelectric point
10.09
Molecular weight
59.30 kDa
Protein length
544 aa Sequence Blast
Gene length
1632 bp Sequence Blast
Function
export of lipid II
Product
lipid II flippase
Essential
no
Synonyms
ytgP

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,073,531 → 3,075,165

Phenotypes of a mutant

  • an amj murJ double mutant is not viable PubMed
  • a sigM murJ double mutant is not viable (due to the lack of both lipid II flippases) PubMed
  • mutants lacking MurJ produce long cells and chains of cells PubMed
  • The protein

    Protein family

    Paralogous protein(s)

    Structure

  • 5T77 (from Thermosipho africanus, 20% identity) PubMed
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • MGNA-A012 (ytgP::erm), available at the NBRP B. subtilis, Japan
  • BKE30050 (ΔmurJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTCTTCATCACCATT, downstream forward: _UP4_TGATGAACTAGATATGATGA
  • BKK30050 (ΔmurJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTCTTCATCACCATT, downstream forward: _UP4_TGATGAACTAGATATGATGA
  • References

    Reviews

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    Original Publications

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